adairАллергология и Иммунология в ПедиатрииAllergology and Immunology in Paediatrics2500-11752712-7958Ассоциация детских аллергологов и иммунологов России10.53529/2500-1175-2024-3-42-53adair-155Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESПоказатели TREC и KREC у пациентов с врожденными пороками сердца. Данные неонатального скринингаTREC and KREC values in patients with congenital heart defects — neonatal screening datahttps://orcid.org/0000-0002-4069-0566БарычеваЛ. Ю.BarychevaL. Yu.

Барычева Людмила Юрьевна — д. м. н., профессор, заведующая кафедрой иммунологии с курсом ДПО 

355017, г. Ставрополь, ул. Мира, д. 310

Lyudmila Yur’evna Barycheva — Dr. Sci., Professor, head of department of Immunology with the course of APE

310 Mira str., Stavropol, 355017

for_ludmila@inbox.ruhttps://orcid.org/0009-0008-7785-4676БачиеваЛ. И.BachievaL. I.

Бачиева Лейла Ибрагимовна — соискатель кафедры иммунологии с курсом ДПО 

355017, г. Ставрополь, ул. Мира, д. 310

Leila Ibragimovna Bachieva — candidate of department of Immunology with course of APE

310 Mira str., Stavropol, 355017

bachleila@mail.ruПучковА. А.PuchkovA. A.

Пучков Андрей Анатольевич — главный врач

355029, г. Ставрополь, ул. Семашко, д. 3

Andrey Anatolyevich Puchkov — Chief Physician of the State Budgetary healthcare Institution 

3 Semashko str., Stavropol, 355029

kdkb@kdkb.ruСелезневаЮ. Г.SeleznevaJ. G.

Селезнева Юлия Геннадьевна — зав. лабораторией неонатального скрининга, врач клинической лабораторной диагностики 

355041, г. Ставрополь, ул. Ломоносова, д. 44

Julia Gennadievna Selezneva — Head of the laboratory of neonatal screening, doctor of clinical laboratory diagnostics of the State Budgetary Healthcare Institution

44 Lomonosov str., Stavropol, 355041

ruta260315@gmail.comhttps://orcid.org/0000-0003-0971-5347КозьмоваН. А.KozmovaN. A.

Козьмова Наталья Александровна — ассистент кафедры иммунологии с курсом ДПО 

355017, г. Ставрополь, ул. Мира, д. 310

Natalia Alexandrovna Kozmova — assistant of department of Immunology with the course of APE 

310 Mira str., Stavropol, 355017

n-kozmova@mail.ruФедеральное государственное бюджетное образовательное учреждение высшего образования «Ставропольский государственный медицинский университет» Министерства здравоохранения Российской Федерации, кафедра иммунологии с курсом ДПОStavropol State Medical UniversityГосударственное бюджетное учреждение тздравоохранения Ставропольского края «Краевая детская клиническая больница»Stavropol Territory Regional Children’s Clinical HospitalГосударственное бюджетное учреждение здравоохранения Ставропольского края «Ставропольский краевой клинический перинатальный центр»Stavropol Regional Clinical Perinatal Center202405102024034253Copyright © Барычева Л.Ю., Бачиева Л.И., Пучков А.А., Селезнева Ю.Г., Козьмова Н.А., 20242024Барычева Л.Ю., Бачиева Л.И., Пучков А.А., Селезнева Ю.Г., Козьмова Н.А.Barycheva L.Y., Bachieva L.I., Puchkov A.A., Selezneva J.G., Kozmova N.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://adair.elpub.ru/jour/article/view/155Актуальность

Актуальность. Дети с врожденными пороками сердца (ВПС) имеют высокий риск инфекционных осложнений с неблагоприятным исходом, что связано с неадекватным иммунным ответом. TREC и KREC являются признанными биомаркерами Т- и В-клеточного лимфопоэза у новорожденных.

Цель исследования

Цель исследования: оценить количество кольцевых участков ДНК — TREC и KREC у детей с врожденными пороками сердца.

Материалы и методы

Материалы и методы. При проведении исследования использовались данные неонатального скрининга на первичные иммунодефициты в Ставропольском крае за период с 01.01.2023 по 30.06.2024. Осуществлен анализ TREC и KREC у 43 новорожденных с ВПС по сравнению со здоровыми младенцами.

Результаты исследования

Результаты исследования. Показатели TREC и KREC у детей с ВПС были ниже, чем у здоровых детей. Установлена связь уровней TREC с абсолютной лимфопенией, развитием инфекционных осложнений. Определено снижение KREC у младенцев с ВПС и генетическими синдромами. Установлено, что уровень TREC менее 650 копий на 105 клеток может быть предиктором развития инфекционных осложнений у новорожденных с ВПС.

Выводы

Выводы: уменьшение числа копий TREC и KREC в неонатальном скрининге позволяет выявить детей с ВПС с высоким риском инфицирования, открывая потенциальные возможности для профилактической терапии. 

Relevance

Relevance. Children with congenital heart disease (CHD) are at high risk of infectious complications with unfavorable outcomes, which is associated with inadequate immune responses. TREC and KREC are recognized biomarkers of T- and B-cell lymphopoiesis.

Objective of the study

Objective of the study: to evaluate the number of circular DNA segments — TREC and KREC in children with congenital heart defects.

Materials and methods

Materials and methods. The study used data from neonatal screening for primary immunodeficiencies in the Stavropol region from January 1, 2023, to June 30, 2024. An analysis of TREC and KREC was conducted in 43 newborns with CHD compared to healthy infants.

Results

Results. The levels of TREC and KREC in children with CHD were lower than in healthy children. A correlation was established between TREC levels and absolute lymphopenia, as well as the development of infectious complications. A decrease in KREC was identified in infants with CHD and genetic syndromes. It was shown that a TREC level of less than 650 copies per 105 cells may be a predictor of the development of infectious complications in newborns with CHD.

Conclusions

Conclusions. The reduction in the number of TREC and KREC copies in neonatal screening allows for the identification of children with CHD at high risk of infection, opening potential opportunities for preventive therapy. 

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The authors declare that there are no conflicts of interest present.